By Howard H. Weetall (auth.), George G. Guilbault, Marco Mascini (eds.)
On may well 4-8, 1987, a NATO complicated examine Workshop at the Analytical makes use of of Immobilized organic Compounds used to be held in Florence, Italy. The Director of the Workshop was once Professor George G. Guilbault of the collage of recent Orleans, and the Co-Director was once Professor Marco Mascini of the college of Florence It vas the aim of this assembly to collect scientists from all NATO international locations with an curiosity in immobilized organic compounds. to debate - tools of immobilization - houses of immobilized compounds - enzyme electrodes and biosensors - optical units using immobilized enzymes - microbial sensors and scientific makes use of of immobilized enzymes - circulation injection research utilizing enzymes - immobilized organic compounds in chemical safeguard detection - pharmaceutical research - makes use of in commercial research - enzyme reactors - pollution detectors - immunosensors - scientific makes use of and purposes - sturdy kingdom and FET sensors targets to be accomplished by means of the convention have been - to allow an alternate of perspectives and adventure in most of these components - to study and severely check the cutting-edge in those fields - to set instructions for destiny study and determine collaborative tasks among scientists in NATO laboratories within the above parts. Thirty-seven lectures got through 36 audio system in the entire above areas.
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Additional resources for Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses
It is reported that enzymes retain up to 80 :Po of their original actiVity when radlation dose lS Kept under 4 mrads. We have used radiation technique to prepare immobillzed urease m bead shaped polymeric gels (4. 5). As shown in Figure 2. urease was first added to the mixture of glass forming monomers. namelg acrylic aCld and methglmethacrylate and polyethyleneglycol as pore making agent. The mixture was then gropped into supercooled petroleum ether. Frozen spheres were lrradiated m a oOCo source to achieve polymerization and copolymerization.
The effects of radiation dose and the ratio of monomers and polyethyleneglycol on enzyme activity were investigated. The results obtained from these studies are given in Table I and II. 75 ITlrads and the ratio of AM mmA and PEG was 401 401 20 respectively. I Cooling UREAl I Water ~----~ ! 19 I ,~ ~--+-~ liquid Nitrogen of IfR1I ~ Cooling Heating PREPARATION of POLYMER SOLUTION i·Radlatlon 60 Co VISCOSITY INCREASE POLYMER SOLUTION by HEATING PREPARATION of ENZYME· POLYMER MIXTURE DROPLET FORMATION ~ • IRRADIATION Figure 2.
MAJOR FEATURES OF THE mETHOD 1. 2. 3. 4. 5. 6. ained Conjugates produced by thIS method are already sterIle 17 C. C. Cuilbault and M. ). Analytical Uses of Immobilized Biological Compounds for Deteclion, Medical and Industrial Uses, 17-23. © 1988 by D. Reidel Publishing Company. ,. / @ Irradiation ~ ...... ,. ,... ~ immobilized product Figure 1. Schematic representations of radiation immobilization technique 7. method is applicable to a varietg of bioagents e. g. cells. hormones. enzgmes and drugs 8.
Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses by Howard H. Weetall (auth.), George G. Guilbault, Marco Mascini (eds.)