Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N.'s Applications of Chimeric Genes and Hybrid Proteins - Part C: PDF

By Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson

ISBN-10: 012182229X

ISBN-13: 9780121822293

The seriously acclaimed laboratory regular for greater than 40 years, Methods in Enzymology is without doubt one of the such a lot hugely revered courses within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless suitable today--truly a necessary book for researchers in all fields of existence sciences

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Additional resources for Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics

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3 j. B. Allen, M. W. Walberg, M. C. Edwards, and S. J. Elledge, Trends Biochem. 20, 511 (1995). 4 p. t . Bartel and S. Fields, in "The Yeast Two-Hybrid System" (P. L. Bartel and S. ), pp. 3-7. Oxford University Press, Oxford, 1997. 5 R. Brent and R. L. , Annu. Rev. Genet. 31, 663 (1997). 6 R. L. Finley, Jr. and R. Brent, Proc. Natl. Acad. Sci. A. 91, 12980 (1994). 7 C. Bendixen, S. Gangloff, and R. Rothstein, Nucleic Acids Res. 22, 1778 (1994). a HIS3 HIS3 A D H l p - L e x A (DBD) A D H l p - L e x A (DBD) 1 5 TRP1 URA3 GALlp-B42 (AD) ADHlp-B42 (AD) 6 Unpublished URA3 L YS2 8LexAop-lacZ 8LexAop-lacZ 5 7 a Key to references: (1) J.

35. John Wiley & Sons, New York, 1997. 8 E. Golemis and I. Serebriiskii, in "Cells: A Laboratory Manual" (D. L. Spector, R. Goldman, and L. ). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1997. 9 M. A. Watson, R. Buckholz, and M. P. Weiner, BioTechniques 21, 255 (1996). 10D. Gietz, A. St. Jean, R. A. Woods, and R. H. Schiestl, Nucleic Acids Res. 20, 1425 (1992). 11 j. Hill, K. A. Ian, G. Donald, and D. E. Griffiths, Nucleic Acids Res. 19, 5791 (1991). ] can vary among colonies, thereby producing an apparent (and misleading) heterogeneity in Bait-mediated transcriptional activation of the two reporters.

BioL 15, 3813 (1995). [2] LexA-BASED TWO-HYBRID SYSTEMS 19 not appreciably activate transcription of the lexA operator-based reporter genes. Results from these control experiments will dictate if the Bait can be used to screen a library, using the initial test conditions, or if modifications of the Bait and/or different combinations of reporter strains/plasmids must be used. Methods. The following protocols utilize basic yeast media and transformation procedures, described in greater detail in Refs.

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Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson


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