By Weihong Tan, Xiaohong Fang
This edited quantity describes cell-SELEX because the primary instrument used to generate aptamer molecules for a variety of functions in molecular medication, bioanalysis and chemical biology. simply built-in into the ordinary heterogeneous mobilephone matrix, aptamers will be successfully utilized in theranostics, bioanalysis, setting detection and biomedical experiences. The ebook gathers experiences that replicate the most recent advances within the box of aptamers, and is composed in fourteen chapters demonstrating crucial examples of those aptamers and aptamer-nanomaterial assemblies, reckoning on the categories of purposes and organic platforms. additionally it is a separate bankruptcy at the usage of aptamers in genuine clinics and what's going to be required to accomplish this crucial target. The publication may be either attractive and valuable to a extensive viewers, together with biologists, bioscientists, and clinicians whose pursuits diversity from chemistry and biomedical engineering to telephone and molecular biology and biotechnology.
Weihong Tan is a uncommon Professor of Chemistry and Biomedical Engineering at Hunan college, China and in addition a school of Florida exotic Professor and V.T. and Louis Jackson Professor of Chemistry on the collage of Florida, USA.
Xiaohong Fang is a Professor on the Institute of Chemistry, chinese language Academy of Sciences, China.
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Nature 346(6287):818–822. 1038/346818a0 3. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249(4968):505–510 4. Lee JF, Stovall GM, Ellington AD (2006) Aptamer therapeutics advance. Curr Opin Chem Biol 10(3):282–289. 015 5. Morris KN, Jensen KB, Julin CM, Weil M, Gold L (1998) High afﬁnity ligands from in vitro selection: complex targets. Proc Natl Acad Sci USA 95(6):2902–2907. 6. 2902 6. Blank M, Weinschenk T, Priemer M, Schluesener H (2001) Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels.
But in practice, the steady progress and good speciﬁcity are not often obtained; if so, the SELEX procedure needs to be adjusted. Cell-SELEX is a highly complex process; many factors can affect the enrichment of aptamer sequences, such as low sequence diversity of DNA library, contamination of PCR reagent, contamination of eluted sequence from target cells (PCR template) by DNA pool or PCR products from previous round of selection, sequence discrimination in PCR, strong nonspeciﬁc binding, low abundance of target molecules on cells, and change of cell states.
06. 001 24. Sefah K, Shangguan D, Xiong X, O’Donoghue MB, Tan W (2010) Development of DNA aptamers using cell-SELEX. Nat Protoc 5(6):1169–1185. 66 25. Hicke BJ, Marion C, Chang YF, Gould T, Lynott CK, Parma D, Schmidt PG, Warren S (2001) Tenascin-C aptamers are generated using tumor cells and puriﬁed protein. J Biol Chem 276 (52):48644–48654. M104651200 26. Liu J, Liu H, Sefah K, Liu B, Pu Y, Van Simaeys D, Tan W (2012) Selection of aptamers speciﬁc for adipose tissue. PLoS ONE 7(5):e37789. 0037789 27.
Aptamers Selected by Cell-SELEX for Theranostics by Weihong Tan, Xiaohong Fang