Download PDF by Weihong Tan, Xiaohong Fang: Aptamers Selected by Cell-SELEX for Theranostics

By Weihong Tan, Xiaohong Fang

ISBN-10: 3662462257

ISBN-13: 9783662462256

ISBN-10: 3662462265

ISBN-13: 9783662462263

This edited quantity describes cell-SELEX because the primary instrument used to generate aptamer molecules for a variety of functions in molecular medication, bioanalysis and chemical biology. simply built-in into the ordinary heterogeneous mobilephone matrix, aptamers will be successfully utilized in theranostics, bioanalysis, setting detection and biomedical experiences. The ebook gathers experiences that replicate the most recent advances within the box of aptamers, and is composed in fourteen chapters demonstrating crucial examples of those aptamers and aptamer-nanomaterial assemblies, reckoning on the categories of purposes and organic platforms. additionally it is a separate bankruptcy at the usage of aptamers in genuine clinics and what's going to be required to accomplish this crucial target. The publication may be either attractive and valuable to a extensive viewers, together with biologists, bioscientists, and clinicians whose pursuits diversity from chemistry and biomedical engineering to telephone and molecular biology and biotechnology.

Weihong Tan is a uncommon Professor of Chemistry and Biomedical Engineering at Hunan college, China and in addition a school of Florida exotic Professor and V.T. and Louis Jackson Professor of Chemistry on the collage of Florida, USA.

Xiaohong Fang is a Professor on the Institute of Chemistry, chinese language Academy of Sciences, China.

Show description

Read or Download Aptamers Selected by Cell-SELEX for Theranostics PDF

Best molecular biology books

Read e-book online Computational Analysis of Biochemical Systems: A Practical PDF

A real knowing of genetic and metabolic functionality and layout is facilitated by means of mathematical and computational tools for studying biochemical platforms. This hands-on reference teaches biochemists and molecular biologists using glossy computational tools for the research of advanced biomedical platforms requiring a modest mathematical heritage.

Daniel J. Park's PCR Protocols (Methods in Molecular Biology) PDF

Recognized for flexibility and robustness, PCR concepts proceed to enhance via various advancements, together with the identity of thermostable DNA polymerases which express quite a number houses to fit given functions. PCR Protocols, 3rd version selects lately constructed instruments and tips, contributed by way of field-leading authors, for the numerous worth that they upload to extra as a rule proven tools.

New PDF release: Microarray Quality Control

Microarray expertise offers researchers within the existence sciences with a innovative instrument for measuring gene expression. besides the fact that, this hugely built method includes a number of steps, from pattern choice to information research, each one at risk of in all probability high priced mistakes. with out sound quality controls, experimental microarrays may well produce lifeless or, even worse, deceptive effects.

Download e-book for iPad: Gametogenesis, Early Embryo Development and Stem Cell by Tiziana A.L. Brevini

This short bargains a concise, convenient evaluate of the most recommendations concerning Embryology, revisited throughout the novel techniques which are utilized day-by-day in stem mobilephone learn and telephone treatment orientated investigations. it really is in line with 3 major parts: -The approach excited by woman gamete differentiation and maturation.

Extra resources for Aptamers Selected by Cell-SELEX for Theranostics

Example text

Nature 346(6287):818–822. 1038/346818a0 3. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249(4968):505–510 4. Lee JF, Stovall GM, Ellington AD (2006) Aptamer therapeutics advance. Curr Opin Chem Biol 10(3):282–289. 015 5. Morris KN, Jensen KB, Julin CM, Weil M, Gold L (1998) High affinity ligands from in vitro selection: complex targets. Proc Natl Acad Sci USA 95(6):2902–2907. 6. 2902 6. Blank M, Weinschenk T, Priemer M, Schluesener H (2001) Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels.

But in practice, the steady progress and good specificity are not often obtained; if so, the SELEX procedure needs to be adjusted. Cell-SELEX is a highly complex process; many factors can affect the enrichment of aptamer sequences, such as low sequence diversity of DNA library, contamination of PCR reagent, contamination of eluted sequence from target cells (PCR template) by DNA pool or PCR products from previous round of selection, sequence discrimination in PCR, strong nonspecific binding, low abundance of target molecules on cells, and change of cell states.

06. 001 24. Sefah K, Shangguan D, Xiong X, O’Donoghue MB, Tan W (2010) Development of DNA aptamers using cell-SELEX. Nat Protoc 5(6):1169–1185. 66 25. Hicke BJ, Marion C, Chang YF, Gould T, Lynott CK, Parma D, Schmidt PG, Warren S (2001) Tenascin-C aptamers are generated using tumor cells and purified protein. J Biol Chem 276 (52):48644–48654. M104651200 26. Liu J, Liu H, Sefah K, Liu B, Pu Y, Van Simaeys D, Tan W (2012) Selection of aptamers specific for adipose tissue. PLoS ONE 7(5):e37789. 0037789 27.

Download PDF sample

Aptamers Selected by Cell-SELEX for Theranostics by Weihong Tan, Xiaohong Fang


by John
4.1

Rated 4.98 of 5 – based on 4 votes