By Nader Hassanzadeh
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The most widely used transposon in Gram-negative bacteria is Tn5, which transposes at high frequency, has relatively little target sequence specificity, and does not share homology with genomic sequences of most bacterial species. However, transposon mutagenesis can have the following technical limitations: The transposase gene must be expressed in the target host, The transposon must be introduced into the host on a suicide vector The transposase gene on the transposon can be expressed in subsequent generations, which may result in genetic instability.
We can recognize four types of bacterial transposon: Insertion Sequences Transposons Composite Transposons Bacteriophage Elements Martin E. Mulligan, 1996-2002 40 Transposable elements IS and Tn Insertion sequences (simple transposons) which carry only one gene. 41 1. Insertion sequences Pieces of DNA that can move in the chromosome. Insertion can cause mutations of a gene or a whole operon. Vehicles are often plasmids and phages. Flanked by inverted repeats (IR). Inner fragment codes for a transposase.
58 Four modes of transposition 59 Mechanisms of Transposition 1. 2. There are two mechanisms by which transposons move: Simple transposition, Replicative transposition 60 Simple transposition Direct or conservative transposition Also known as direct or conservative transposition involves the transfer of the element from one place to another. The original element is excised from the original site leaving a double-strand break behind. The original molecule is irreparably damaged as a result.
Bacteria Genetics by Nader Hassanzadeh